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Recombinant Protein Services

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Medicilon is a leading provider of comprehensive, high quality recombinant protein and bioprocess services. We offer a variety of recombinant protein expression platforms along with a host of other protein services like chemical protein synthesis, protein refolding and structural biology services.

E. coli Expression System

E. coli expression system is the earliest developed and most widely used gene expression system. In recent decades, E.coli expression system has been popularly used and developed for producing a wide variety of different types of proteins. Compared with other expression systems, E. coli is a well-established host with the advantages of short culturing time, easy genetic manipulation and low cost media.

Medicilon researchers established a well-developed E. coli expression and purification services platform, which offers the expression and purification services of a variety of recombinant proteins, with a fast turn-around time and competitive pricing.

Service:

  1. Generation of Recombinant Plasmid
  2. Expression Test in Host
  3. Soluble Expression Test
  4. Small Scale Expression and Purification (2L Cell Culture)
  5. Scale Up Expression and Purification
Steps Items Timeline Comments
1 Generation of Recombinant Plasmid 2 weeks Multiple expression vector, different affinity tag: His, Trx , GST, SUMO…
2 Expression of Recombinant Protein 1 week Multiple expression host
3 Soluble Expression Optimization 1-2 weeks Test different induction concentration, timepoint, temperatures or reconstruct plasmids
4 Small Scale Expression and Purification 1-2 weeks Purification of soluble expression or from inclusion body
5 Scale Up Expression and Purification TBD Culture in flask or fermentor

Remarks:

  1. The service is highly flexible to meet your request. One or more above steps could be separately provided.
  2. Affinity purification, ion exchange purification, hydrophobic beads purification and size exclusion column purification or their combination will be used for protein purification.

Yeast Expression System


Yeast expression system contains the advantages of both prokaryotic and eukaryotic system, such as easy scaling up, high expression level, proper protein folding, fast growth rate and low cost, which makes them widely used in industry and research field.

Saccharomyces cerevisiae and the methylotrophic yeast Pichia pastoris are commonly used expression strain. Pichia pastoris is more popular, especially in the large-scale production of eukaryotic recombinant proteins, due to the fast growth rate, multiple commercial expression vectors and high efficiency of secretion expression.

Medicilon provides a well-developed yeast expression and purification services, including the mini-scale and large scale expression and purification of recombinant protein in Pichia pastoris and Saccharomyces cerevisiae.



Service:

  1. Recombinant Plasmid Generation for Yeast Expression
  2. Transformation of Linear Construct
  3. Verification of Positive Transformants by PCR and Western Blot Method
  4. Small Scale Expression and Purification (2L Cell Culture)
  5. Scale Up Expression and Purification of Recombinant Protein (10L or more)
Steps Items Timeline
1 Recombinant Plasmid Generation 2 weeks
2 Transformation of Linear Construct 1 week
3 Verification of Positive Transformants via PCR 3-4weeks
4 Small Scale Expression and Purification 2-4 weeks
5 Scale Up Expression and Purification TBD

Remarks:

  1. The service is highly flexible to meet your request. One or more above steps could be separately provided.
  2. Affinity purification, ion exchange purification, hydrophobic beads purification and size exclusion column purification or their combination will be used for protein purification.

Baculovirus Expression Vector System


Baculovirus expression vector system (BEVS) is a power system to produce a variety of prokaryotic and eukaryotic protein.  It is also an efficient tool for preparing vaccines.

The system could effectively produce high yield target protein with proper folding and post-translation modifications in an intracellular or secretion expression means.

Medicilon researchers establish a well-developed baculovirus-insect cell expression services platform. MedicilonWe provide the expression and purification services on preparation of recombinant baculovirus and recombinant protein. We have a good track record in producing kinase and recombinant protein complexes.



Service:

  1. Generation of recombinant Bacmid DNA
  2. Preparation of recombinant baculovirus
  3. Titration of baculovirus
  4. Protein expression verification and optimization
  5. Small scale expression and purification of recombinant protein in insect cell
  6. Scale up expression and purification of recombinant protein in insect cell
Steps Items Timeline Comments
1 Generation of recombinant Bacmid DNA 3 weeks Different affinity tag: GST tag, His tag, Flag tag…..
2 Preparation of recombinant baculovirus 2 weeks
3 Titration of baculovirus 2-3 days Quantitative-PCR or based on gp64 antibody
4 Protein expression verification and optimization 2 weeks MOIs, PIs or different cell lines
5 Small Scale expression and purification 2-4 weeks 500 ml-2L expression in flask
6 Scale up expression and purification TBD Culture in flask or fermentor

Remarks:The service is highly flexible to meet your request. One or more above steps could be separately provided.

Mammalian Expression

  • CHO, Vero, BHK, 293 cells etc
  • Adherence and Suspension Culture
  • Transient Expression Screen
  • Stable Cell Line Generation

Rabbit IgG expressed in transient transfected 293 cells

Note: The condition medium was run in 10%SDS-PAGE gel and stained with coomassie blue

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