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Discovery Biology Services

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Our Discovery Biology department offers services supporting structure-based drug discovery from determination of novel targets to final structures. Our platform is one of the earliest established structural biology platforms in China and has been certified by the Shanghai Government.

Biochemical Assay

Technology Platforms

  • Fluorescence: Lance; Alphascreen; Z’-LYTE ; HTRF,TR-FRET,FP etc
  • Luminescence: Kinase-Glo; ADP-Glo etc
  • Absorbance: UV; VI

Kinase Assay: VEGFR2 I


Protease Assay: Renin inhibition using FRET


Validated Biochemical Assay List

Validated Enzymatic Targets
Abl COX-1/COX-2 HER2 PI3K alpha
AKT1 C-Raf IKK beta PI3K beta
AKT2 DPPIV JAK1 PI3K delta
AKT3 EGFR JAK2 PI3K gamma
ALK EGFR (L858R,T790M) JAK3 PKA
Aurora A Factor Xa LCK PKC delta
Aurora B FGFR1 Lipoxygenase PKC gamma
B-Raf Flt1/VEGFR1 Lyn Plk1
BTK Flt3 MAPK14 20S proteasome
Cathepsin K/L/B/S Flt4 MARK1 Renin
CDK/P25 α- Glucosidase MEK1 SAHase
CHK1 β- Glucosidase MEK2 SGK1
CHK2 GSK3 beta mTOR SRC
c-Kit H+/K+ ATPase PDE5 Syk
c-Met HDAC1 &HDACs PDGFR beta VEGFR2
Note: The enzyme in blue is Kinase

The enzyme in blue is Kinase

Cellular-based Assay

Technology Platforms

  • Cytotoxicity Assay
  • Apoptosis Assay
  • Cell Migration Assay
  • Cell Invasion Assay
  • Immunostaining Assay
  • Immunofluorescence Assay (96/384 wells)
  • Reporter Gene Assay (GFP/Luc)
  • Cellular Uptake assay
  • Gene Knockdown Assay
  • RNAi Assay
  • MicroRNA Over-expression Assay
  • MicroRNA Knockdown Assay
  • Adenovirus/retrovirus/lentivirus

Cytotoxicity Assay Using celltiter glo


Cell-based Cathepsin Assay for irreversible inhibitors


Cell Cycle Analysis Using Flow Cytometry


Western Blot Analysis

Expression level of Phospho-Akt and Akt in mouse tumor



Migration Assay in HT1080 cells and MDA-MB-231 cells

Cytotoxicity Assay Using celltiter glo


Cell-based Cathepsin Assay for irreversible inhibitors


Pre-migration

Post-migration


Background

Positive control






Radioactive Assay

Technology Platforms (3H, 33P, 32P, 35S, 125I,14C)

  • Radioactive receptor binding assay
  • Radioligand binding assay
  • Radioactive uptake assay
  • Cell proliferation assay (3H-TdR)
  • Radioimmunoassay (RIA)
  • Immunoradiometric assay (IRMA)

Receptor binding assay-3H


3H-5HT uptake assay-3H


Radioactive iodine uptake assay-125I

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